In vitro Production of PPV-free Sweet cherry (Prunus avium cv. Siahe-Mashhad) by Meristem culture and micro-grafting

Naddaf, Mohammad Esmaeil; Rabiei, Gholamreza; Ganji Moghadam, Ebrahim; Mohammadkhani, Abdorahman

doi:10.22034/jpbb.2021.282382.1005

Document Type : Original Article

Authors

¹ Ph.D. Student, Department of Horticultural Science, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

² Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

³ Associate Professor, Department of Horticultural Science, Khorasan Razavi Agriculture and Natural Resources Research & Education Center, Mashhad, Iran

https://doi.org/10.22034/jpbb.2021.282382.1005

Abstract

Plum Pox Virus (PPV) is one of the most common viral agents in sweet cherry. This study was carried out to investigate the effect of culturing method and explant type for production of PPV-free plants in sweet cherry cv. Siahe-Mashhad, in a factorial experiment based on a completely randomized design (CRD). The first factor was the culturing method in two levels (A: Meristem culture and B: Micro-grafting) and the second factor, the type of explants in four levels (M1S1: 5mm and in vivo explant, M1S2: 2mm and in vivo explant, M2S1: 2mm and in vitro explant and M2S2: 5mm and in vitro explant). Explants of shoot tips were cultured for meristem culture as well as micro-grafting on the 'Gissela 6' rootstock and then cultured in a MS medium containing 1 mg.l^-lBAP in controlled conditions. The results showed that among the types of explant in both survival and health plantlet indices was significant, but there was a significant difference in the culturing method only in the survival plantlet index and their interaction (at 5% level). The highest percentage of survival plantlet index (48.6%) was recorded for meristem culture when explant was 5 mm in vitro and the highest percentage of healthy planted index (41.6%) was obtained for meristem culture when 2 mm in vitro explants was used. The results of percentage of healthy plantlets in total detection of PPV using Enzyme-linked immunosorbent assay (ELISA) test showed that 30.5% of all regenerated plants in both cultures were PPV- free.

Graphical Abstract

Keywords

Main Subjects

Plant Cell, Organ and Tissue Culture

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Journal of Agricultural Sciences and Engineering

In vitro Production of PPV-free Sweet cherry (Prunus avium cv. Siahe-Mashhad) by Meristem culture and micro-grafting

References

References

Volume 3, Issue 1
January 2021
Pages 51-59

In vitro Production of PPV-free Sweet cherry (Prunus avium cv. Siahe-Mashhad) by Meristem culture and micro-grafting

References

References

Volume 3, Issue 1January 2021Pages 51-59

Volume 3, Issue 1
January 2021
Pages 51-59